Psycho-Babble Neurotransmitters Thread 984996

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Are we getting closer? Assaying the proteome.

Posted by SLS on May 10, 2011, at 9:30:42

Common Proteomic Changes in the Hippocampus in Schizophrenia and Bipolar Disorder and Particular Evidence for Involvement of Cornu Ammonis Regions 2 and 3

Archives of General Psychiatry, 05/10/2011

Focking M et al. Overall, similar protein changes were observed in schizophrenia and bipolar disorder and for the first time indicate that the most prominent proteomic changes occur within the hippocampus in cornu ammonis regions 2 and 3. The cytoskeletal protein septin 11 and the cellular trafficking process of clathrinmediated endocytosis are implicated by study.
Methods

A comparative proteomic study, including validation of differential expression, was performed.

Midhippocampus samples from wellmatched groups of 20 subjects with schizophrenia, 20 subjects with bipolar disorder, and 20 control cases from the Stanley Medical Research Institute Array Collection were analyzed.

Results

Across all hippocampal regions, 108 protein spots in schizophrenia and 165 protein spots in bipolar disorder were differentially expressed compared with controls.

Sixtyone proteins were differentially expressed in both disorders. One hundred fiftytwo of these proteins were identified by mass spectrometry, and they implicated a range of different processes including cytoskeletal and metabolic functions.

In both disorders, cornu ammonis regions 2 and 3 were affected to a significantly greater degree than other hippocampal regions.

Additionally, numerous proteins showed expression changes in more than 1 region and more than 1 disorder.

Validation work confirmed changes in septin 11 and in the expression of proteins involved in clathrinmediated endocytosis in both schizophrenia and bipolar disorder

- Scott

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