Posted by morganpmiller on June 24, 2009, at 15:21:30
In reply to Re: N-acetylcysteine and OCD, posted by mogger on May 7, 2009, at 11:47:45
> I am trying N-acetylcysteine for OCD. I was wondering if its effects are purely the glutamate modulation or does it also affect sodium and calcium channels?
> moggerIf you want something that modulates glutamate you may want to try a good resveratrol product:
Resveratrol Increases Glutamate Uptake, Glutathione Content, and S100B Secretion in Cortical Astrocyte Cultures
JournalCellular and Molecular NeurobiologySubject CollectionBiomedical and Life Sciences
SpringerLink DateThursday, June 07, 2007Original paper
Resveratrol Increases Glutamate Uptake, Glutathione Content, and S100B Secretion in Cortical Astrocyte CulturesLúcia Maria Vieira de Almeida1, Cristopher Celintano Piñeiro1, Marina Concli Leite1, Giovana Brolese1, Francine Tramontina1, Ana Maria Feoli2, Carmem Gottfried1 and Carlos-Alberto Gonçalves1 Contact Information
(1) Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600 anexo, Porto Alegre, 90035-003, RS, Brazil
(2) Faculdade de Enfermagem, Nutrição e Fisioterapia, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, BrazilReceived: 4 November 2006 Accepted: 16 April 2007 Published online: 7 June 2007
Abstract Resveratrol (3,5,4′-trihydroxy-trans-stilbene) is a polyphenol present in grapes and red wine, which has antioxidant properties and a wide range of other biological effects. In this study, we investigated the effect of resveratrol, in a concentration range of 10250 μM, on primary cortical astrocytes; evaluating cell morphology, parameters of glutamate metabolism such as glutamate uptake, glutamine synthetase activity and glutathione total content, and S100B secretion. Astrocyte cultures were prepared of cerebral cortex from neonate Wistar rats. Morphology was evaluated by phase-contrast microscopy and immunocytochemistry for glial fibrillary acidic protein (GFAP). Glutamate uptake was measured using l-[2,3-3H]glutamate. Glutamine synthetase and content of glutathione were measured by enzymatic colorimetric assays. S100B content was determined by ELISA. Typical polygonal morphology becomes stellated when astrocyte cultures were exposed to 250 μM resveratrol for 24 h. At concentration of 25 μM, resveratrol was able to increase glutamate uptake and glutathione content. Conversely, at 250 μM, resveratrol decreased glutamate uptake. Unexpectedly, resveratrol at this high concentration increased glutamine synthetase activity. Extracellular S100B increased from 50 μM upwards. Our findings reinforce the protective role of this compound in some brain disorders, particularly those involving glutamate toxicity. However, the underlying mechanisms of these changes are not clear at the moment and it is necessary caution with its administration because elevated levels of this compound could contribute to aggravate these conditions.
poster:morganpmiller
thread:887620
URL: http://www.dr-bob.org/babble/alter/20090410/msgs/902971.html